Search results for "molecular marker"
showing 10 items of 62 documents
Molecular markers and endometrial cancer
2022
None
Expression Profile of Endoglin in Different Grades of Endometrial Cancer
2019
Background Endoglin is a marker of active, proliferating endothelial cells of blood vessels. In many cancers, it is present in both peripheral vessels and vessels located inside the tumor. Endoglin is more specific and sensitive compared to other tumor angiogenesis markers. It is suggested that endoglin can be considered a reliable marker of disease outcome. Objective The aim of the study was to assess the expression of endoglin and to determine its potential usefulness as a complementary molecular marker of endometrial cancer. Method The study included 60 women who underwent hysterectomy: 45 with endometrioid endometrial cancer (study group) and 15 without neoplastic changes (control group…
Estimation of the degrading genetic potential of soil bacterial communities: a new tool for evaluating and predicting soil contamination by organic m…
2007
During the last two centuries, industrialisation has led to increasing contamination of the environment by xenobiotics, notably aromatic compounds (i.e. PAHs, pesticides). These widespread pollutants are a major threat affecting soil quality and human health. In this context, numerous policies have been drawn up notably in Europe, to monitor the threats of pollution to ecosystems and to detect and implement measures to counterbalance the damage. Nevertheless, the European Commission and scientific community are still confronted with a lack of appropriate tools to monitor biodiversity in relation to environmental processes affecting global warming and soil protection. This study proposes too…
Infection by Endosymbiotic “Male-Killing” Bacteria in Coleoptera
2018
Wolbachia, Rickettsia, Spiroplasma and Cardinium are endosymbiotic and intracellular bacteria known to cause numerous disorders in host reproduction, reflected in their common name “male-killers”. In this study, 297 beetle species from various taxonomic groups were screened with the use of molecular markers for the presence of infection by any of these endosymbionts. Wolbachia was found to be the most common “male-killer” among beetle hosts as it infected approx. 27% of species. Rickettsia, Spiroplasma and Cardinium were much less prevalent as they infected: 8%, 3% and 2%, respectively, of the studied beetle species. This is the first report of Cardinium presence in beetle hosts. Incidences…
Crocus sativus L. Ecotypes from Mediterranean Countries: Phenological, Morpho-Productive, Qualitative and Genetic Traits
2021
The characterization of C. sativus ecotypes is of great interest for preserving them from a possible genetic erosion due to the decrease of European cultivation surface. In this study, we evaluated four ecotypes from Italy (Sardinia and Abruzzo), Spain (Castilla-La Mancha), and Greece (Kozani) in order to detect the existence of variability and promote the biodiversity of this crop. Thirty-one traits related to saffron flowering, flower morphology, production of spice and daughter corms, vegetative development (leaf and corm traits), and spice quality, were evaluated. In addition, a genetic analysis through three PCR-based approaches, SSRs, RAPD, and SRAP was assessed. Results highlighted a…
New polymorphic markers for genetic diversity studies in an invasive plant: the common ragweed (Ambrosia artemisiifolia L.)
2014
Common ragweed is an annual herb that belongs to the Asteraceae family. It is known as an invasive plant originating from the USA. In France the common ragweed is especially abundant in the Rhône-Alpes basin and currently spreads northwards in Burgundy. Ragweed colonizes different types of environments, such as railways, river sides, wastelands, farmlands and cultivated crops (especially sunflowers crops). Herbicide resistance to linuron, glyphosate and/or acetolactate synthase inhibitors has been reported in the USA, which complicates the chemical control of ragweed. In addition, the highly allergenic pollen of ragweed causes severe allergies. These elements make this plant a major threat …
The TP53 colorectal cancer international collaborative study on the prognostic and predictive significance of p53 mutation: influence of tumor site, …
2005
Purpose The aims of the TP53 Colorectal Cancer (CRC) International Collaborative Study were to evaluate the possible associations between specific TP53 mutations and tumor site, and to evaluate the prognostic and predictive significance of these mutations in different site, stage, and treatment subgroups. Patients and Methods A total of 3,583 CRC patients from 25 different research groups in 17 countries were recruited to the study. Patients were divided into three groups according to site of the primary tumor. TP53 mutational analyses spanned exons 4 to 8. Results TP53 mutations were found in 34% of the proximal colon tumors and in 45% of the distal colon and rectal tumors. They were assoc…
Environmental effects on molecular and phenotypic variation in populations of Eruca sativa across a steep climatic gradient
2013
Abstract In Israel Eruca sativa has a geographically narrow distribution across a steep climatic gradient that ranges from mesic Mediterranean to hot desert environments. These conditions offer an opportunity to study the influence of the environment on intraspecific genetic variation. For this, we combined an analysis of neutral genetic markers with a phenotypic evaluation in common-garden experiments, and environmental characterization of populations that included climatic and edaphic parameters, as well as geographic distribution. A Bayesian clustering of individuals from nine representative populations based on amplified fragment length polymorphism (AFLP) divided the populations into a…
A contribution to the phylogeny and biogeography of the genus Isoetes (Isoetaceae, Lycopodiidae) in the Mediterranean region
2019
To better understand the phylogeny of Isoetes species in the Mediterranean region, sequences of the second intron of a LFY-like homolog were determined for six populations of three Mediterranean Isoetes species and analyzed with other LFY and ITS sequences of Euro-Mediterranean species available in GenBank. Nomenclature of selected species was updated according to recent literature. Investigations of voucher specimens from previously sequenced taxa revealed that a specimen of I. histrix from Crete, Greece used in several previous molecular studies is probably I. phrygia. Analysis of the limited LFY and ITS DNA sequence data presented here agrees with the organization of Mediterranean specie…
SNP markers for black-grass (Alopecurus myosuroides Huds.) genotypes resistant to Acetyl CoA-carboxylase inhibiting herbicides
2002
Chloroplastic acetyl CoA-carboxylase (ACCase) is the target of widely used, specific graminicide herbicides: cyclohexanediones (CHDs) and aryloxyphenoxypropionates (APPs). Resistance to these compounds is a worldwide, increasing problem. Population genetic studies aimed at understanding the dynamics of this situation and the diffusion of resistance genes within and between weed populations are challenging because biological assays are not adequate for this purpose, and because different mechanisms of resistance confer a similar resistance phenotype. Molecular markers for specifically detecting resistance genes are therefore urgently needed to conduct such studies. For this purpose, we clone…